During a routine transfer of a convicted federal witness, US Marshals Lee Scott and Chase Daniels make a stopover in Bunkie, Louisiana after experiencing some car troubles. Stopping in at a local diner to figure out their next move they are offered room and board for the night by the hospitable Mama Jo and her "daughters". Once at the house a series of events start to unfold as Mama Jo and her brewed aren't exactly what they appear to be as it increasingly becomes a fight for survival as Lee, Chase, and the convict Gator are separated and tortured as they are prepared to be sacrificed for their sins. With the help of one, they will fight to survive the night and escape the hell house that's holding them captive as what lurks behind the last door could be the death of them all.